产品货号：
JN2100
中文名称：
SARS-CoV-2刺突蛋白三聚体
英文名称：
SARS-CoV-2 S-trimer Protein(C-6His)
产品规格：
1mg
发货周期：
1～3天
产品价格：
询价
本制品由我们的哺乳动物表达系统制备，目的基因编码SARS-CoV-2的S蛋白(Cys15-Gln1208)，C端带有6*His标签。
Accession:QHD43416.1
来源：人源细胞
储存液：PBS，PH 7.4，0.2μm过滤
纯化方法：亲和层析纯化
用途：免疫原、检测标准品
保存：≤-20℃
运输：干冰运输
产品稳定性：
Stability testing is performed in the product buffer to see whether different temperatures affect the antigen binding.The result showed that product bioactivity is no significant differences after storage for 14 days at 4℃ or 25℃.
分子量：
生物活性（ELISA）：
生物活性（BLI）：
Flow cytometry:
The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process.Most notable is severe acute respiratory syndrome (SARS).The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion.It is also a major immunogen and a target for entry inhibitors.It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor.The spike protein is a large type I transmembrane protein containing two subunits,S1 and S2.S1 mainly contains a receptor binding domain (RBD),which is responsible for recognizing the cell surface receptor.S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses,as well as protective immunity.
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Accession:QHD43416.1
来源：人源细胞
储存液：PBS，PH 7.4，0.2μm过滤
纯化方法：亲和层析纯化
用途：免疫原、检测标准品
保存：≤-20℃
运输：干冰运输
产品稳定性：
Stability testing is performed in the product buffer to see whether different temperatures affect the antigen binding.The result showed that product bioactivity is no significant differences after storage for 14 days at 4℃ or 25℃.
 |  |
| Bind with Recombinant Human ACE-2 (C-Fc)(Cat#JN2061) | Bind with Anti-SARS-CoV-2 S1 mAb (5D9)(Cat#JN4025) |
分子量：
 |  |
| Greater than 95% as determined by reducing SDS-PAGE. | SARS-CoV-2 S-trimer Protein(C-6His)(Cat#JN2100) molecular mass was tested by SEC-MALS around 550～670kDa and the purity was more than 90%. |
生物活性（ELISA）：
 |  |
| Immobilized SARS-CoV-2 S-trimer(Cat#JN2100) at 2μg/ml (100μL/well) can bind Human ACE-2 (C-Fc)(Cat#JN2061).The ED50 for this effect is 15～120ng/ml. | Immobilized SARS-CoV-2 S-trimer(Cat#JN2100) at 2μg/ml (100μL/well) can bind Anti-SARS-CoV-2 S1 mAb (5D9)(Cat#JN4025).The ED50 for this effect is 5～30ng/ml. |
生物活性（BLI）：
 | |
| Loaded Human ACE-2-Fc(Cat#JN2061) on Protein A Biosensor,can bind SARS-CoV-2 S-trimer Protein-His(Cat#JN2100) with an affinity constant of 0.125 nM as determined in BLI assay. | Loaded Anti-SARS-CoV-2 S1 mAb (5D9)(Cat#JN4025) on Protein A Biosensor,can bind SARS-CoV-2 S-trimer Protein-His(Cat#JN2100) with an affinity constant of 3.88 nM as determined in BLI assay. |
 | |
| Loaded SARS-CoV-2 S-trimer Protein-His(Cat#JN2100) on HIS1K Biosensor,can bind Anti-SARS-CoV-2 S-hIgG1 Antibody (8A5)(Cat#JN4034) with an affinity constant of 1.11 nM as determined in BLI assay. |
Flow cytometry:
 | |
| SARS-CoV-2 S-trimer Protein (C-6His)(Cat#JN2100) can bind 293-ACE2 Overexpressed Cells. The percentage for positive cell is 92.1%. |
The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process.Most notable is severe acute respiratory syndrome (SARS).The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion.It is also a major immunogen and a target for entry inhibitors.It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor.The spike protein is a large type I transmembrane protein containing two subunits,S1 and S2.S1 mainly contains a receptor binding domain (RBD),which is responsible for recognizing the cell surface receptor.S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses,as well as protective immunity.
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